Journal: bioRxiv

Article Title: Functional inactivation of the telomerase chaperone TCAB1 primes cells for the activation of ALT in osteosarcoma

doi: 10.64898/2026.01.02.691864

Figure Lengend Snippet: A) Schematic representation of the orientation of the TP53 and WRAP53/TCAB1 genes within the genome. B) Representative image of an agarose gel from a TRAP assay performed on OS tumors. SJSA1 serves as a telomerase positive control and buffer alone serves as the negative control. IC; internal PCR control, Telomerase positive; +, Telomerase negative; -. C) Representative TEL-FISH result for SJSA1(negative control) and indicated OS tumors. Arrows indicate cells with ultra bright telomeric foci. D) Representative TP53-IHC result for SJSA1 (expresses mutant TP53) and OS tumors with complete loss of TP53 expression. E) TCAB1-IHC result for SJSA1(positive control) and indicated OS tumors with loss of TCAB1 expression.

Article Snippet: For p53, slides were steamed in pH6.0 citrate-based unmasking solution (Cat# H-3300, Vector Laboratories) for 30 min, blocked with ReadyProbes Endogenous HRP and AP Blocking Solution (Invitrogen, #R37629) and Protein Block (#ab64226, abcam), then were incubated overnight at 4C in mouse monoclonal primary antibody against p53 (1:50 dilution, Cat# sc-126, Santa Cruz Biotechnology).

Techniques: Agarose Gel Electrophoresis, TRAP Assay, Positive Control, Negative Control, Control, Mutagenesis, Expressing

Journal: bioRxiv

Article Title: Functional inactivation of the telomerase chaperone TCAB1 primes cells for the activation of ALT in osteosarcoma

doi: 10.64898/2026.01.02.691864

Figure Lengend Snippet: A) Categorical heatmap summarizes the SVs for TP53, TCAB1, and ALT associated genes that were identified for each of the five OS tumors analyzed by WGS. Complex (COM) and translocations (TRA) are represented as dark blue and light blue respectively. B) Illustration of the specific SVs identified in each of the five OS tumors analyzed by WGS. C) PCR analysis conducted on the 3.6 KB flanking region between TP53 and TCAB1 that identifies deletion of this locus in tumors 0560-000 and CPRIT 1981. Red lines represent location of primers D) Validation of the translocation event between JARID2-TCAB1 on Ch6:17 in the tumor OS39. HEK293 serves as a negative control for T6:17.

Article Snippet: For p53, slides were steamed in pH6.0 citrate-based unmasking solution (Cat# H-3300, Vector Laboratories) for 30 min, blocked with ReadyProbes Endogenous HRP and AP Blocking Solution (Invitrogen, #R37629) and Protein Block (#ab64226, abcam), then were incubated overnight at 4C in mouse monoclonal primary antibody against p53 (1:50 dilution, Cat# sc-126, Santa Cruz Biotechnology).

Techniques: Biomarker Discovery, Translocation Assay, Negative Control

Journal: bioRxiv

Article Title: Functional inactivation of the telomerase chaperone TCAB1 primes cells for the activation of ALT in osteosarcoma

doi: 10.64898/2026.01.02.691864

Figure Lengend Snippet: A) Categorical heatmap summarizes ALT status, SVs for TP53, TCAB1, and ALT associated genes that were identified for all OS tumors analyzed by WGS. B) Relative percentage of ALT- and ALT+ samples with a SV affecting the TP53 and TCAB1 genomic regions are represented as a stacked bar chart where deletions, inversions, translocations and complex events are orange, yellow, light blue and dark blue respectively. C) Categorical heatmap summarizes the functional inactivation of TCAB1 and ALT status across all 38 samples analyzed by either IHC or WGS. D) Theoretical framework for the evolution of telomerase and ALT positive tumors during osteosarcomagenesis.

Article Snippet: For p53, slides were steamed in pH6.0 citrate-based unmasking solution (Cat# H-3300, Vector Laboratories) for 30 min, blocked with ReadyProbes Endogenous HRP and AP Blocking Solution (Invitrogen, #R37629) and Protein Block (#ab64226, abcam), then were incubated overnight at 4C in mouse monoclonal primary antibody against p53 (1:50 dilution, Cat# sc-126, Santa Cruz Biotechnology).

Techniques: Functional Assay

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Down Expression of Zyxin is Associated with Down Expression of p53 in Colorectal Cancer

doi: 10.22088/IJMCM.BUMS.14.1.462

Figure Lengend Snippet: Primer sequences used in Real-Time PCR

Article Snippet: In the next step, the nitrocellulose membrane was incubated with mouse monoclonal antibody against ZYX (1:1000 dilution, Sigma-Aldrich, USA, Z0377), mouse monoclonal antibody against P53 (1:2000 dilution, STJ, USA, STJ96954) and mouse monoclonal antibody against beta-actin (1:1000 dilution, Santa Cruz sc-47778) overnight at 4°C.

Techniques: Sequencing

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Down Expression of Zyxin is Associated with Down Expression of p53 in Colorectal Cancer

doi: 10.22088/IJMCM.BUMS.14.1.462

Figure Lengend Snippet: ZYX and p53 gene expression levels were determined by qRT-PCR. The relative mRNA expression of (A) ZYX and (B) p53, fold changes in gene expression were expressed as mean ± SD.

Article Snippet: In the next step, the nitrocellulose membrane was incubated with mouse monoclonal antibody against ZYX (1:1000 dilution, Sigma-Aldrich, USA, Z0377), mouse monoclonal antibody against P53 (1:2000 dilution, STJ, USA, STJ96954) and mouse monoclonal antibody against beta-actin (1:1000 dilution, Santa Cruz sc-47778) overnight at 4°C.

Techniques: Gene Expression, Quantitative RT-PCR, Expressing

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Down Expression of Zyxin is Associated with Down Expression of p53 in Colorectal Cancer

doi: 10.22088/IJMCM.BUMS.14.1.462

Figure Lengend Snippet: The protein levels of ZYX and P53 in tumor tissues (n=5) and non-cancerous tissues (n=5) as determined by Western blotting. The Band intensity of the studied proteins was assessed using ImageJ software (A) and expressed as mean ± SD for (B) ZYX and p53. Columns with **** or ** represent significant difference (P<0.05) compared with tumor margin.

Article Snippet: In the next step, the nitrocellulose membrane was incubated with mouse monoclonal antibody against ZYX (1:1000 dilution, Sigma-Aldrich, USA, Z0377), mouse monoclonal antibody against P53 (1:2000 dilution, STJ, USA, STJ96954) and mouse monoclonal antibody against beta-actin (1:1000 dilution, Santa Cruz sc-47778) overnight at 4°C.

Techniques: Western Blot, Software

Journal: International Journal of Molecular Sciences

Article Title: Early and Late Effects of Low-Dose X-ray Exposure in Human Fibroblasts: DNA Repair Foci, Proliferation, Autophagy, and Senescence

doi: 10.3390/ijms25158253

Figure Lengend Snippet: Changes in the number of DNA repair foci in fibroblasts exposed to X-ray radiation at doses of 100 and 2000 mGy: ( a ) γH2AX; ( b ) 53BP1; ( c ) pATM; and ( d ) p-p53. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with corresponding control. ( e ) Representative microphotographs of immunofluorescently stained cell nuclei of fibroblasts post irradiation (2000 mGy, 0.5 h) showing γH2AX (red), 53BP1 (green), pATM (green), and p-p53 (green) foci. DAPI counterstaining is shown in blue.

Article Snippet: The following primary antibodies were used: rabbit monoclonal antibodies against γH2AX (phospho S139) (dilution 1:800, clone EP854(2)Y, Abcam, Waltham, MA, USA); mouse monoclonal antibodies against 53BP1 (dilution 1:400, clone BP13, Merck-Millipore, Burlington, VT, USA); mouse monoclonal antibodies against phosphorylated ATM (phospho S1981) protein (dilution 1:200, clone 10H11.E12, Abcam, Waltham, MA, USA); mouse monoclonal antibodies against phospho-p53 (Ser15) (16G8) (dilution 1:400, Cell Signaling Technology, Danvers, MA, USA); mouse monoclonal antibodies against Ki-67 protein (dilution 1:400, clone Ki-S5, Merck-Millipore, Burlington, VT, USA); rabbit polyclonal antibody against LC3BI/II proteins (dilution 1:400, cat. no. ABC929, Merck-Millipore, Burlington, VT, USA).

Techniques: Control, Staining, Irradiation

Journal: International Journal of Molecular Sciences

Article Title: Early and Late Effects of Low-Dose X-ray Exposure in Human Fibroblasts: DNA Repair Foci, Proliferation, Autophagy, and Senescence

doi: 10.3390/ijms25158253

Figure Lengend Snippet: Changes in the integrated fluorescence of immunofluorescently stained DNA repair foci in fibroblasts exposed to X-ray radiation at doses of 100 and 2000 mGy: ( a ) γH2AX; ( b ) 53BP1; ( c ) pATM; and ( d ) p-p53. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with corresponding control.

Article Snippet: The following primary antibodies were used: rabbit monoclonal antibodies against γH2AX (phospho S139) (dilution 1:800, clone EP854(2)Y, Abcam, Waltham, MA, USA); mouse monoclonal antibodies against 53BP1 (dilution 1:400, clone BP13, Merck-Millipore, Burlington, VT, USA); mouse monoclonal antibodies against phosphorylated ATM (phospho S1981) protein (dilution 1:200, clone 10H11.E12, Abcam, Waltham, MA, USA); mouse monoclonal antibodies against phospho-p53 (Ser15) (16G8) (dilution 1:400, Cell Signaling Technology, Danvers, MA, USA); mouse monoclonal antibodies against Ki-67 protein (dilution 1:400, clone Ki-S5, Merck-Millipore, Burlington, VT, USA); rabbit polyclonal antibody against LC3BI/II proteins (dilution 1:400, cat. no. ABC929, Merck-Millipore, Burlington, VT, USA).

Techniques: Fluorescence, Staining, Control

Journal: International Journal of Molecular Sciences

Article Title: Early and Late Effects of Low-Dose X-ray Exposure in Human Fibroblasts: DNA Repair Foci, Proliferation, Autophagy, and Senescence

doi: 10.3390/ijms25158253

Figure Lengend Snippet: Changes in the number of DNA repair foci depending on the post-irradiation passage number in the control and X-ray-exposed fibroblasts: ( a ) γH2AX; ( b ) 53BP1; ( c ) pATM; and ( d ) p-p53 (Ser-15). * p < 0.05 compared with corresponding control.

Article Snippet: The following primary antibodies were used: rabbit monoclonal antibodies against γH2AX (phospho S139) (dilution 1:800, clone EP854(2)Y, Abcam, Waltham, MA, USA); mouse monoclonal antibodies against 53BP1 (dilution 1:400, clone BP13, Merck-Millipore, Burlington, VT, USA); mouse monoclonal antibodies against phosphorylated ATM (phospho S1981) protein (dilution 1:200, clone 10H11.E12, Abcam, Waltham, MA, USA); mouse monoclonal antibodies against phospho-p53 (Ser15) (16G8) (dilution 1:400, Cell Signaling Technology, Danvers, MA, USA); mouse monoclonal antibodies against Ki-67 protein (dilution 1:400, clone Ki-S5, Merck-Millipore, Burlington, VT, USA); rabbit polyclonal antibody against LC3BI/II proteins (dilution 1:400, cat. no. ABC929, Merck-Millipore, Burlington, VT, USA).

Techniques: Irradiation, Control

Journal: Oncology Letters

Article Title: Analysis of the clinicopathological features of tailgut cyst with emphasis on the development of neoplastic lesions

doi: 10.3892/ol.2024.14419

Figure Lengend Snippet: Histopathological and immunohistochemical features (Patient 4). (A) Papillotubular invasive neoplastic growth is observed (blue arrows). A non-invasive dysplastic area is also observed (black arrows). (B) Invasion of fatty tissue by adenocarcinoma (magnification, ×20; scale bars, 500 µm). (C) Adenocarcinoma cells are columnar in shape and have large round to oval nuclei. (D) Dysplastic cells have mildly to moderately enlarged nuclei (black arrows) (hematoxylin and eosin staining). (E) p53 is diffusely expressed in the nuclei of adenocarcinoma cells. (F) Cytoplasmic cytokeratin 20-positive carcinoma cells are scattered (magnification, ×200; scale bars, 50 µm).

Article Snippet: Sections were incubated with a rabbit monoclonal antibody against caudal type homeobox 2 (CDX2) (cat. no. EPR2764Y; pre-diluted; Roche Diagnostics), a mouse monoclonal antibody against cytokeratin (CK)-7 (cat. no. OV-TL12/30; pre-diluted; DAKO; Agilent Technologies, Inc.), a mouse monoclonal antibody against CK-20 (cat. no. Ks20.8; pre-diluted; DAKO; Agilent Technologies, Inc.) and a mouse monoclonal antibody against p53 (cat. no. DO-7; diluted 1:50; DAKO; Agilent Technologies, Inc.) for 20 min at room temperature.

Techniques: Immunohistochemical staining, Staining